Antileishmanial activity of 4-phenyl-1-[2-(phthalimido-2-yl)ethyl]-1H-1,2,3-triazole (PT4) derivative on Leishmania amazonensis and Leishmania braziliensis: In silico ADMET, in vitro activity, docking and molecular dynamic simulations.

Organic compounds obtained by click chemistry reactions have demonstrated a broad spectrum of biological activities being widely applied for the development of molecules against pathogens of medical and veterinary importance. Cutaneous leishmaniasis (CL), caused by intracellular protozoa parasite of genus Leishmania, comprises a complex of clinical manifestations that affect the skin and mucous membranes. The available drugs for the treatment are toxic and costly, with long periods of treatment, and the emergence of resistant strains has been reported. In this study we investigated the in vitro effects of a phthalimide-1,2,3-triazole derivative, the 4-Phenyl-1-[2-(phthalimido-2-yl)ethyl]-1H-1,2,3-triazole (PT4) obtained by click chemistry, on mammalian cells and on L. amazonensis and L. braziliensis, the causative agents of CL in Brazil. In silico ADMET evaluation of PT4 showed that this molecule has good pharmacokinetic properties with no violation of Lipinski's rules. The in vitro assays showed that PT4 was more selective for both Leishmania species than to mammalian cells. This compound also presented low cytotoxicity to mammalian cells with CC50 > 500 µM. Treatment of promastigote forms with different concentrations of PT4 resulted in ultrastructural alterations, such as plasma membrane wrinkling, shortening of cell body, increased cell volume and cell rupture. The molecular dynamic simulations showed that PT4 interacts with Lanosterol 14 α-demethylase from Leishmania, an essential enzyme of lipid synthesis pathway in this parasite. Our results demonstrated PT4 was effective against both species of Leishmania. PT4 caused a decrease of mitochondrial membrane potential and increased production of reactive oxygen species, which may lead to parasite death. Taken together, our results pointed PT4 as promissing therapeutic agent against CL.

Perfil e hábitos alimentares dos usuários da trofoterapia no Recanto Madre Paulina, Petrolina/PE / El perfil y los hábitos dietéticos de los usuarios en la Trofoterapia Esquina Madre Paulina, Petrolina / Profile and dietary habits of users in the Corner Trofotherapy Mother Pauline, Petrolina

Identificar o perfil e os hábitos alimentares dos usuários do Recanto Madre Paulina (Petrolina/PE). Métodos:Trata-se de um estudo descritivo com abordagem quantitativa, desenvolvido a partir de um questionário aplicado a 41 usuários da instituição, de ambos os sexos e acima de 20 anos. Os dados foram organizados e tabulados usando o programa Microsoft Excel 2007. Resultados: 12% dos usuários procedem de Petrolina; 70% são do sexo feminino; a maioria é adepto do catolicismo (57%); 50% estão na faixa etária entre 40 e 60 anos; a auto-imagem é negativa para 59% e 62% apresentam IMC acima do normal; conhecem os riscos da alimentação desregulada (95%), praticam restrição na dieta (24%), tem hábitos alimentares saudáveis (36%). Conclusão: A trofoterapia pode mudar a concepção dos usuários quanto à qualidade dos alimentos e de seus hábitos alimentares. Ressalta-se a necessidade de propostas educativas em segurança alimentar.

Objective: To identify the profile and feeding habits of users of Mother Pauline Corner (Petrolina / PE). Methods: This is a descriptive study with quantitative approach, developed from a questionnaire administered to 41 users of the institution, and of both sexes over 20 years. Data were organized and tabulated using the Microsoft Excel 2007. Results: 12% of users come from Petrolina, 70% are female, most are adherent of Catholicism (57%), 50% are aged between 40 and 60 years, the self-image is negative for 59% and 62 % have a BMI above the normal; know the risks of unregulated power supply (95%), practice restriction in the diet (24%), have healthy eating habits (36%). Conclusion: Trofoterapia can change the design of the users on the quality of food and eating habits. We stress the need for proposals for education in food safety.

Objetivo: Identificar los hábitos de alimentación y el perfil de los usuarios de la Madre Paulina Rincón (Petrolina / PE). Métodos: Se realizó un estudio descriptivo con enfoque cuantitativo, desarrollado a partir de un cuestionario aplicado a 41 usuarios de la institución, y de ambos sexos de más de 20 años. Los datos fueron organizados y tabulados utilizando el Microsoft Excel 2007. Resultados: 12% de los usuarios proceden de Petrolina, el 70% son mujeres, la mayoría son adherentes de la religión católica (57%), el 50% tienen edades comprendidas entre los años 40 y 60, la auto-imagen es negativa para un 59% y el 62 % tiene un IMC superior al normal; conocer los riesgos del suministro de energía no regulada (95%), la restricción de la práctica en la dieta (24%), la alimentación saludable (36%). Conclusión: Trofoterapia puede cambiar el diseño de los usuarios sobre la calidad de los alimentos y hábitos alimenticios. Hacemos hincapié en la necesidad de presentar propuestas para la educación en inocuidad de los alimentos.

Comparison of self-report and interview administration methods based on the Brazilian versions of the Western Ontario Rotator Cuff Index and Disabilities of the Arm, Shoulder and Hand Questionnaire in patients with rotator cuff disorders.

OBJECTIVE: The purpose of the present study was to compare self-report and interview administration methods using the Western Ontario Rotator Cuff Index (WORC) and Disabilities of the Arm, Shoulder and Hand Questionnaire (DASH) in patients with rotator cuff disorders. METHODS: Thirty male and female patients over 18 years of age with rotator cuff disorders (tendinopathy or rotator cuff tear) and Brazilian Portuguese as their primary language were recruited for assessment via administration of the Western Ontario Rotator Cuff Index and and Disabilities of the Arm, Shoulder and Hand Questionnaire. A randomization method was used to determine whether the questionnaires would be self-reported (n=15) or administered by an interviewer (n=15). Pearson correlation coefficients were used to evaluate the correlation between the Western Ontario Rotator Cuff Index and and Disabilities of the Arm, Shoulder and Hand Questionnaire in each group. The t-test was used to determine whether the difference in mean questionnaire scores and administration time was statistically significant. For statistical analysis, the level of significance was set at 5%. RESULTS: The mean subject age was 55.07 years, ranging from 27 to 74 years. Most patients had a diagnosis of tendinopathy (n=21). With regard to level of schooling, the majority (n=26) of subjects had completed a college degree or higher. The mean questionnaire scores and administration times did not significantly differ between the two groups (p>0.05). There were statistically significant correlations (p<0.05) between Western Ontario Rotator Cuff Index and and Disabilities of the Arm, Shoulder and Hand Questionnaire, and strong correlations were found between the questionnaires in both groups. CONCLUSION: There are no differences between the Western Ontario Rotator Cuff Index and Disabilities of the Arm, Shoulder and Hand Questionnaire administration methods with regard to administration time or correlations between the questionnaires.

Comparison of self-report and interview administration methods based on the Brazilian versions of the Western Ontario Rotator Cuff Index and Disabilities of the Arm, Shoulder and Hand Questionnaire in patients with rotator cuff disorders

OBJECTIVE: The purpose of the present study was to compare self-report and interview administration methods using the Western Ontario Rotator Cuff Index (WORC) and Disabilities of the Arm, Shoulder and Hand Questionnaire (DASH) in patients with rotator cuff disorders. METHODS: Thirty male and female patients over 18 years of age with rotator cuff disorders (tendinopathy or rotator cuff tear) and Brazilian Portuguese as their primary language were recruited for assessment via administration of the Western Ontario Rotator Cuff Index and and Disabilities of the Arm, Shoulder and Hand Questionnaire. A randomization method was used to determine whether the questionnaires would be self-reported (n=15) or administered by an interviewer (n=15). Pearson correlation coefficients were used to evaluate the correlation between the Western Ontario Rotator Cuff Index and and Disabilities of the Arm, Shoulder and Hand Questionnaire in each group. The t-test was used to determine whether the difference in mean questionnaire scores and administration time was statistically significant. For statistical analysis, the level of significance was set at 5 percent. RESULTS: The mean subject age was 55.07 years, ranging from 27 to 74 years. Most patients had a diagnosis of tendinopathy (n=21). With regard to level of schooling, the majority (n=26) of subjects had completed a college degree or higher. The mean questionnaire scores and administration times did not significantly differ between the two groups (p>0.05). There were statistically significant correlations (p<0.05) between Western Ontario Rotator Cuff Index and and Disabilities of the Arm, Shoulder and Hand Questionnaire, and strong correlations were found between the questionnaires in both groups. CONCLUSION: There are no differences between the Western Ontario Rotator Cuff Index and Disabilities of the Arm, Shoulder and Hand Questionnaire administration methods with regard to administration time or correlations...

Efeito de tratamento cirúrgico sobre a atividade da enzima hepática lecitina: colesterol aciltransperase (LCAT)na esquistossomose mansônica / Surgical treatment effect on the lecitin liver enzyme activity: cholesterol acyltransferase (LCAT) in schistosomiasis mansoni

A esquistossomose mansônica é uma doença tropical que constitui um importante problema de saúde pública, na Regiäo Nordeste do Brasil, onde é encontrada em alta endemicidade. Essa parasitose tem o fígado como principal alvo de suas lesöes histológicas, alteraçöes fisiopatológicas e manifestaçöes clínicas. Estudos anteriores reportam alteraçöes no metabolismo lipídico associadas à forma hepatoesplênica da esquistosomose.Uma das principais alteraçöes consiste na reduçäo da atividade da enzima hepática LCAT, responsável pela esterificaçäo do colesterol no plasma. Neste trabalho, avaliamos a atividade da LCAT no plasma de pacientes portadores da esquistossomose mansônica hepatoesplênica, os quais foram submetidos a esplenectomia e reimplante de parte de tecido do baço. A atividade enzimática da LCAT foi determinada com substrato radioativo. O [14C]colesterol livre e esterificado, formados por açäo da LCAT, foram separados por cromatografia em camada delgada e a radioatividade das amostras foi contada em analisador de cintilaçäo líquida. A atividade da LCAT nos pacientes submetidos a esplenectomia e reimplante de tecido do baço apresentou reduçäo de 32 por cento, em relaçäo ao grupo controle. Contudo, nos portadores da doença que näo foram submetidos ao procedimento cirúrgico a reduçäo na atividade da LCAT foi o dobro (64 por cento) da observada em pacientes esplenectomizados e com reimplante de parte do tecido do baço. Esses resultados sugerem haver uma melhora significativa no efeito da forma grave da esquistossomose mansônica sobre a atividade da LCAT.

Atualizacao de alguns tumores de celulas germinativas do ovario e dos tumores derivados dos cordoes sexuais e estroma / Updating of some ovarian germ cell tumors and tumors derived from sexual corneas and stroma

O ovario e o orgao da especie humana que apresenta maior variedade de tumores. Tendo em vista a grande quantidade de heterogeneidade de tumores que o acometem, as neoplasias ovarianas foram fruto de numerosas classificacoes emitidas por varios autores, ate que em 1973, a Organizacao Mundial da Saude (OMS) oficializou a classificacao histogenica por SEROO, SCULLY e SOBIN, a qual foi referendada pela Federacao Internacional de Ginecologia e Obstetricia (FIGO). Esta classificacao histogenetica foi a que mais satisfez os ginecologistas, oncologistas e principalmente os patologistas, pois usando criterios que levavam em conta a evolucao morfogenetica da gonada, conseguia caracterizar a grande maioria dos tumores que afetam o orgao. Analisamos, retrospectivamente, atraves de revisao histologica, 9 tumores de células germinativas e 9 tumores dos cordões sexuais do ovário, obtidos através de levantamento nos arquivos do serviço de Patologia no Laboratório de Anatomia Patológica e Citopatológica de Bauru, bem como a observação da incidência desses tumores na cidade de Bauru. Foram obtidos 241 casos de tumores ovarianos no periodo compreendido entre janeiro de 1989 a outubro de 1997, dos quais, 42 caos são de tumores malignos (17,42%), 196 benignos (81,32%) e 3 classificados como borderline (1,24%). Selecionamos 18 (7,46%) de tumores pouco comuns que incluem alguns tumores das células germinativas e outros do cordão sexual e do estroma. Realizamos revisão e atualização destes tumores do ovário em seus varios aspectos como: incidência, caracterização clínica, anatomia patológica, tratamento e prognóstico, mostrando semelhante incidência desses tumores nesta região com os observados na literatura.